Revolutionizing membrane protein overexpression in bacteria
نویسندگان
چکیده
منابع مشابه
Revolutionizing membrane protein overexpression in bacteria
The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, especially for eukaryotic membrane proteins. This has initiated a revolution of membrane protein o...
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A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used "Walker strains" C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression. For unknown reasons, overexpression of many membrane proteins in these strains is hardly toxic, often resulting in h...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2009
ISSN: 1751-7915
DOI: 10.1111/j.1751-7915.2009.00148.x